Fig. 2: Induced senescent human cell lines show cell-type-specific responses to senolytic treatment. | Nature Communications

Fig. 2: Induced senescent human cell lines show cell-type-specific responses to senolytic treatment.

From: Systematic profiling reveals distinct senescence signatures and regulators across human brain cell types

Fig. 2: Induced senescent human cell lines show cell-type-specific responses to senolytic treatment.

A Schematic depicting mechanism of action of senolytics Dasatinib + Quercetin and Navitoclax on anti-apoptotic pathways active in senescent cells (Created in BioRender. Plessis-Belair, J. (2025) https://BioRender.com/lgfnmsj). B Z-score heatmap showing anti-apoptotic marker expression (MCL1, BCL2, ABL1, BCL2L1) in all cell lines (astrocytes—green, endothelial cells—orange, microglia—purple, oligodendrocytes—yellow, neurons—brown) based on bulk RNAseq following 7-day DMSO (blue) or BrdU (pink) treatment. C–G Normalized percent cell survival of astrocytes (C), endothelial cells (D), microglia (E), oligodendrocytes (F), and neurons (G) following 7-day DMSO (grey) and 100 µM BrdU (red) treatment with 48-h dasatinib (D) + quercetin (Q) treatment at concentrations of 50 nM D + 10 µM Q and 100 nM D + 20 nM Q (n = 3 replicates). H–L Normalized percent cell survival of astrocytes (H), endothelial cells (I), microglia (J), oligodendrocytes (K), and neurons (L) following 7-day DMSO (grey) and 100 uM BrdU (red) treatment with 48-h navitoclax treatment at concentrations of 1 µM, 10 µM, and 20 µM (n = 3 replicates). Data in (C–L) analyzed by two-way ANOVA with Šídák’s multiple comparisons test. All graphs show mean with standard deviation (ns, p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001). Source data and exact p-values are provided as a Source Data file.

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