Fig. 6: The olfactory information relay in the olfactory bulb is impaired in Trim66 knockout mice.

a Schematic illustration of the experimental paradigm. Optetrodes were lowered into the mitral cell layer (MCL) to record the local field potential (LFP) and spikes of mitral or tufted cells. This experiment was performed using adult mice. b Representative raw traces and filtered Beta and High gamma oscillations in the LFP signal in respond to odors recorded from one Trim66 +/− and one Trim66 −/− mouse. c Cumulative probability and comparison of the normalized power for odor-evoked Beta band in the two groups. Trim66 +/−, n = 48 animal-odor pairs; Trim66 −/−, n = 36 animal-odor pairs. d Cumulative probability and comparison of the normalized power for odor-evoked High gamma band in the two groups. Trim66 +/−, n = 48 animal-odor pairs; Trim66 −/−, n = 36 animal-odor pairs. e, f Raster plots and peristimulus time histogram of the odor-evoked inhibitory responses and odor-evoked excitatory responses in Trim66 +/− group (e) and Trim66 −/− group (f). g, h Cumulative probability of the mean firing rate (MFR) for all odor-evoked excitatory responses (g) and inhibitory responses (h). Inserted graphs show changes in the firing rate of odor-evoked excitatory responses (g, n = 79 and 34 unit–odor pairs for the Trim66 +/− and Trim66 −/− group) and inhibitory responses (h, n = 179 and 26 unit–odor pairs for the Trim66 +/− and Trim66 −/− group). Data were shown as box plots (c, d, g, h), which were employed to summarize the data distribution, depicting the median, first and third quartiles (Q1 and Q3), and the non-outlier range. The central line in the box indicates the median, while the box edges represent Q1 and Q3. The whiskers extend to the minimum and maximum non-outlying values. Potential outliers, defined as values exceeding 1.5 times the interquartile range (IQR) are shown as individual data points. A comparison of the two groups was carried out using a two-sample K–S test, followed by a two-sided Wilcoxon rank-sum test (c, d, g, h). The p values are shown. Source data are provided as a Source Data file.