Fig. 1: Syngap1 levels do not affect the number of progenitor cells in mice.
From: Syngap1 and the development of murine neocortical progenitor cells
a RNAscope for Syngap1 (red) and Pax6 (gray) in the somatosensory cortex of wild-type mice at E18.5. Low and top lines represent the limits of the ventricular zone (VZ) and cortical plate (CP), respectively. Boxed area at higher magnification on the right. Circles highlight the cells that show co-expression of Syngap1 and Pax6 in the VZ. These results were confirmed in three independent mice. b Overview of midbrain coronal sections of control and homozygous Syngap1 knockout (Syngap1KO) mice analyzed by PAX6 immunolabeling at E18.5. Lines represent the limits of the ventricle and CP. c Control (CT), heterozygous (+/−) and homozygous (−/−) Syngap1KO brains analyzed by PAX6 and TBR2 immunolabeling in the somatosensory cortex at E18.5 (n = 3 mice/genotype; one-way ANOVA Dunnett’s multiple comparisons test: PAX6 – CT vs −/− p = 0.8805, CT vs +/− p = 0.7600, F2,6 = 0.2097; TBR2 – CT vs −/− p = 0.2206, CT vs +/− p = 0.6551, F2,6 = 1.507). d Cortical thickness in the somatosensory cortex of Control (CT), heterozygous (+/−), and homozygous (−/−) Syngap1KO brains at E18.5 (n = 3 mice/genotype; one-way ANOVA Dunnett’s multiple comparisons test: CT vs −/− p = 0.2708; CT vs +/− p = 0.6740, F2,6 = 1.242). DL deep layer; UL upper layer, cc corpus callosum; Values are mean ± SEM; ns not significant; Scale bars: 50 µm (lower magnification), 10 µm (higher magnification), 500 µm (b, overview). Source data are provided as a Source data file.
