Fig. 4: Representation of light position in the dIPN and the habenula.

a Illustration of experimental setup of presentation of frontal visual stimulus to embedded fish. b Illustration of somata distribution in the dIPN as seen in the Tg(elavl3:H2BGCaMP6s) line. c Tuning of dIPN cells to light position. Only reliably responding ROIs are shown, each ROI is colored according to the position it is tuned to. Left, data from multiple fish registered to one another (n = 7). Right, same as in the left panel but for four individual fish. The dIPN in each dataset is marked with a dashed circle. d Illustration of dIPN somata and neuropil. e Tuning of dIPN neuropil to landmark position. Left, average tuning map of Tg(s1168t:Gal4; UAS:GCaMP6s) fish, showing the average response pattern, each pixel is colored according to the direction that it is tuned to (n = 14). Right, example fish tuning maps of the dIPN in three example fish. f Illustration of the zebrafish brain, the habenula is highlighted in pink. g Tuning of habenula cells to landmark position as found in Tg(elavl3:H2B-GCaMP6s) and Tg(elavl3:GCaMP6s) fish. Only reliably responding ROIs are shown, each ROI is colored according to the position it is tuned to. Left, data from multiple fish morphed to one another (n = 16). Right, same as in the left panel but for four individual fish. The left habenula in each dataset is marked with a dashed circle. h Responses of eight neurons from the left habenula to the appearance of a light bar in the eight possible locations. For each neuron the mean ± sem response is shown. Orange shading indicates stimulus presentation.