Fig. 10: GLP-1R–VAPB/SPHKAP interactions and GLP-1R-dependent mitochondrial remodelling in primary human islets. | Nature Communications

Fig. 10: GLP-1R–VAPB/SPHKAP interactions and GLP-1R-dependent mitochondrial remodelling in primary human islets.

From: GLP-1R associates with VAPB and SPHKAP at ERMCSs to regulate β-cell mitochondrial remodelling and function

Fig. 10

a PLA showing interactions between endogenous GLP-1R and VAPB (left) or SPHKAP (right) in exendin-4 (Ex-4)-stimulated primary human islets; size bars, 20 μm. Images representative of n = 3 biologically independent experiments (islets from independent donors). b Negative control (no primary antibody) for PLA data shown in (a); nuclei (DAPI), blue; size bar, 20 µm. c Confocal microscopy analysis of mitochondrial morphology in vehicle (Veh) versus Ex-4-stimulated primary human islets; mitochondria labelled with MitoTracker Red; size bars, 20 μm; inset, magnification areas; images representative of n = 5 biologically independent experiments (islets from independent donors). d Percentage of human islet cells classified as presenting hyperfused, tubular or fragmented mitochondria per condition; n = 5 biologically independent experiments, p values as indicated by two-way ANOVA with Tukey post-hoc test. Data are mean ± SEM.

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