Fig. 6: GLP-1R/SPHKAP-dependent mitochondrial remodelling. | Nature Communications

Fig. 6: GLP-1R/SPHKAP-dependent mitochondrial remodelling.

From: GLP-1R associates with VAPB and SPHKAP at ERMCSs to regulate β-cell mitochondrial remodelling and function

Fig. 6

a Confocal microscopy analysis of mitochondrial morphology in vehicle (Veh) versus exendin-4 (Ex-4)-stimulated WT INS-1 832/3 cells; top, representative images with mitochondria labelled with MitoTracker Red; size bars, 10 μm; bottom, percentage of cells classified as presenting hyperfused, tubular or fragmented mitochondria per condition; n = 4 biologically independent experiments, p values as indicated by two-way ANOVA with Tukey post-hoc test. b As in (a) for INS-1 832/3 GLP-1R KO cells; n = 4 biologically independent experiments; p values as indicated by two-way ANOVA with Tukey post-hoc test. c As in (a) for Control versus SPHKAP RNAi-treated INS-1 832/3 cells under Veh versus Ex-4-stimulated conditions; n = 5 biologically independent experiments, p values as indicated by two-way ANOVA with Tukey post-hoc test. d Maximum intensity projections (MIP) from confocal z-stacks of mitochondria in vehicle (Veh) versus Ex-4-stimulated Control and SPHKAP RNAi-treated INS-1 832/3 cells; size bars, 10 µm; images representative of n = 3 biologically independent experiments. e Mitochondrial morphology in Control versus SPHKAP RNAi-treated INS-1 832/3 cells under Veh versus Ex-4-stimulated conditions, imaged by super-resolved STED microscopy; size bars, 2 μm. Images representative of n = 3 biologically independent experiments. Data are mean ± SEM.

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