Fig. 8: SPHKAP control of GLP-1R function in primary mouse islets. | Nature Communications

Fig. 8: SPHKAP control of GLP-1R function in primary mouse islets.

From: GLP-1R associates with VAPB and SPHKAP at ERMCSs to regulate β-cell mitochondrial remodelling and function

Fig. 8

a PLA showing interactions between endogenous GLP-1R and VAPB (left) or SPHKAP (right) in exendin-4 (Ex-4)-stimulated primary mouse islets; size bars, 20 μm; images representative of n = 3 biologically independent experiments. b Ex-4-induced changes in ATP levels per total protein in lysates from Control versus SPHKAP shRNA-transduced primary mouse islets; n = 5 biologically independent experiments, p value as indicated by one-way ANOVA with Šidák post-hoc test. c Ex-4-induced changes in thapsigargin-triggered ROS accumulation per cell in Control versus SPHKAP shRNA-transduced primary mouse islets; n = 6 biologically independent experiments, p value as indicated by one-way ANOVA with Šidák post-hoc test. d Ex-4-induced potentiation of insulin secretion in Control versus SPHKAP shRNA-transduced primary mouse islets; n = 9 biologically independent experiments, p values as indicated by one-way ANOVA with Šidák post-hoc test; fold over 11 mM glucose also shown, p value as indicated by two-tailed ratio paired t-test. Data are mean ± SEM.

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