Fig. 9: SPHKAP control of GLP-1R-dependent mitochondrial remodelling in primary mouse islets. | Nature Communications

Fig. 9: SPHKAP control of GLP-1R-dependent mitochondrial remodelling in primary mouse islets.

From: GLP-1R associates with VAPB and SPHKAP at ERMCSs to regulate β-cell mitochondrial remodelling and function

Fig. 9

a Confocal microscopy analysis of mitochondrial morphology in vehicle (Veh) versus exendin-4 (Ex-4)-stimulated WT primary mouse islets; top, representative images with MitoTracker Red-labelled mitochondria; size bars, 20 μm; bottom, percentage of islet cells classified as presenting hyperfused, tubular or fragmented mitochondria per condition; n = 5 biologically independent experiments (islet preps from separate mice), p values as indicated by two-way ANOVA with Tukey post-hoc test. b As in (a) for islets from GLP-1R KO mice; n = 5 biologically independent experiments; p values as indicated by two-way ANOVA with Tukey post-hoc test. c As in (a) for Control versus SPHKAP shRNA-transduced mouse islets under Veh versus Ex-4-stimulated conditions; n = 6 biologically independent experiments, p values as indicated by two-way ANOVA with Tukey post-hoc test. d As in (a) for Control versus SPHKAP RNAi-treated dispersed primary mouse islet cells under Veh versus Ex-4-stimulated conditions; size bars, 5 μm; n = 4 biologically independent experiments, p values as indicated by two-way ANOVA with Tukey post-hoc test. Data are mean ± SEM.

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