Fig. 4: Influenza A virus NS1 tempers ADP-ribosylation by the host.
From: Global remodeling of ADP-ribosylation by PARP1 suppresses influenza A virus infection
A NS1 suppresses infection-induced ADP-ribosylation throughout infection. Proteins and PARylation were detected by blotting lysates from human lung A549 cells infected with PR8 (MOI = 5) or PR8∆NS1 (MOI = 5) or mock infected. B Virus-specific differences in ADP-ribosylation map to NS1 and require its RNA-binding activity. A549 cells were infected with PR8∆NS1 (MOI = 4.5) and co-infected with virus encoding the indicated heterotypic NS1 or the RNA-binding mutant NS1 K38A/R41A (MOI = 0.5). Infections proceeded for 8 h prior to lysis and blotting. Migration positions of molecular weight markers (kDa) are indicated. Source data are provided as a Source Data file.
