Fig. 1: Distinct intrinsic properties of L2/3 pyramidal neurons in the peritumoral cortex of brain tumor patients with and without seizures. | Nature Communications

Fig. 1: Distinct intrinsic properties of L2/3 pyramidal neurons in the peritumoral cortex of brain tumor patients with and without seizures.

From: Aberrant neural activity in the peritumoral cortex underlies the progression of tumor-associated seizures

Fig. 1: Distinct intrinsic properties of L2/3 pyramidal neurons in the peritumoral cortex of brain tumor patients with and without seizures.The alternative text for this image may have been generated using AI.

a Example MRI images aligned with intra-operative neuronavigation for tissues resected from two patients, locations of tissue removal indicated in green areas. b Hematoxylin-eosin (HE) staining of PTC from an anaplastic oligodendroglioma patient. Zone I indicates the peritumoral cortex with clear cortical layers. Zone II indicates the tumor area with giant nuclei (inset), mitoses, abnormal vasculature, and high cell density (right panel). Zone III with HE (bottom left) and Ki67 (bottom right) staining indicate the tumor invasion zone. c Scheme of experimental design. Peritumoral neurons in the PTC slices are recorded with whole-cell patch-clamp techniques. Right pie charts indicate distribution of tumor types within the cohort and anatomical sites of tissue resection. Gr WHO grade, Oligo oligodendroglioma, Astro astrocytoma, GBM glioblastoma. d Recorded cells are filled with biocytin and reconstructed using confocal imaging (staining used are streptavidin, magenta; SMI 32, yellow; GFAP, blue). Image shows L2/3 (left cells) and L5/6 pyramidal cells (right cells). Right panel: a higher magnification of the L2/3 pyramidal cell from dashed box. Inset: infrared differential interference contrast (IR-DIC) image showing the same cell and attached patch-clamp pipette. e Left: example voltage traces of a L2/3 pyramidal cells in response to incremental current injections. Right panels: distinct resting membrane potential (P = 0.0005) and input resistance (P = 0.0057) of L2/3 PCs from patients with TAS (red, n = 122 cells) and without TAS (grey, n = 55 cells). f Left: example voltage traces showing maximum firing frequency of TAS (red) and non-TAS PCs (grey). Right panel: quantifications of spike frequencies in response to depolarizing currents in TAS (red, n = 122 cells) and non-TAS groups (grey, n = 53 cells, linear regression with comparison of slope). Inset shows the maximum frequencies for both groups. Error bars show mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. ns not significant, PTC peritumoral cortex, AP action potential, PC pyramidal cell, TAS tumor-associated seizures. Statistics: e, two-sided Mann–Whitney test. f linear regression with comparison of slope. Source data are provided as a Source Data file.

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