Fig. 6: MPC-medicated immune cell activities.
From: Microbial Product Cocktails for Personalized Cancer Immunotherapy
Cell populations expressing functional intracellular cytokines IFN-γ, TNF-α, and perforin (PRF) are reported as the percentage of A myeloid cells (CD45⁺CD11b⁺), B NK cells (CD45⁺NK1.1⁺), C CD4⁺ T cells (CD45⁺CD3⁺CD4⁺), and D CD8⁺ T cells (CD45⁺CD3⁺CD8⁺). Error bars represent the mean ± SE. P values were derived using the Kruskal-Wallis test followed by Dunn’s test. CI 95%. ns P > 0.05; * P < 0.05; ** P < 0.01. Mouse biological replicates n = 6 for PBS, n = 9 for BCG, and n = 10 for MPC17 (A-D). E Schematic of the GNR-LNA biosensor for single cell gene expression analysis. F Relative expression levels of IFN-γ and TNF-α. G Representative confocal images of IFN-γ and TNF-α expression. Scale bar, 10 μm. P values were derived using either one-sided Welch T test or Kruskal-Wallis Test. Outliers ( > 1.5×IQR) excluded for statistics. ** P < 0.01; *** P < 0.001. Statistics were calculated with mouse biological replicates n = 5 for Control, BCG, and MPC17 (E-G). Source data are provided as a Source Data file.
