Fig. 10: Importin β binds both Ran-GTP and βIBB in the presence of RanBP1.
From: Ran modulates allosteric crosstalk between importin β surfaces
A Solvent surface representations of importin β bound to αIBB (yellow ribbon, left), Ran-GTP alone (magenta, center), and Ran-GTP:RanBP1 (magenta and orange, right). Semitransparent blue circles labeled I, II, and III highlight three binding regions in importin β. B Native gel electrophoresis shows a sequential addition of Ran-GTP (lane 3) and Ran-GTP:RanBP1 (lane 4) to purified importin β (lane 2) along with a titration of αIBB (lanes 5–12) against a preformed importin β:Ran-GTP:RanBP1 complex. Lane 1 has molecular weight (M.W.) native gel markers. The MBP-αIBB control is in lane 13, although this fusion protein is basic and does not migrate well on a native acrylamide gel. The quantification of the native gel in B is presented in Supplementary Fig. 10A. Source data, including all native gels, are provided as a Source data file.
