Fig. 3: Cryo-EM structure of importin β:αIBB bound to FG-repeats.
From: Ran modulates allosteric crosstalk between importin β surfaces
A Representative 2D class averages (top) and 3D structure of the importin β:αIBB:FG complex (bottom). Importin β is colored blue, the αIBB is yellow, and FGs are red. The final 3.21 Å cryo-EM density (gray) is overlaid onto the refined model for FG-peptides in chicken-wire style, and around the αIBB as a solid surface. B Schematic diagram of the FG-binding pockets on the outer surface of importin β. FH5 and FH5’ create a bipartite binding site. C Schematic diagram of the importin β residues (colored blue) that surround FGs (colored red) in pocket 1 (left), pocket 4 (center), and pocket 5 (right).
