Fig. 6: Cryo-EM structure of the importin β:Ran-GDP:RanBP1 complex.
From: Ran modulates allosteric crosstalk between importin β surfaces
A Representative 2D class averages (top) and the 3D structure of the importin β:Ran-GDP:RanBP1 complex (bottom). Importin β is colored blue, Ran-GDP is light magenta, and RanBP1 is orange. The final 3.21 Å cryo-EM density (gray) of Ran-GDP:RanBP1 is overlaid onto the refined model. B Schematic diagram of importin β contacts with Ran-GDP:RanBP1 within a 4.5 Å distance. Notably, only HEAT repeats 1–10 are visible in the reconstruction. C Overlay of Ran-GDP:RanBP1 and Ran-GTP:RanBP1 achieved by superimposing importin β HEAT repeats 1–10 from the two experimentally determined ternary complexes, namely importin βːRan-GTP:RanBP1 (Fig. 5B) and importin βːRan-GDP:RanBP1 (Fig. 6B).
