Fig. 2: S. hominis AH5011 encodes a plasmid-borne daptide bacteriocin.

a Spot-on-lawn bioactivity of AH5011 and plasmid-cured mutants against a lawn of AH4553, with inhibition zone diameters represented by black dashed lines. Red dashed line indicates the limit of detection. Scale bar = 10 mm. Data represents the mean of n = 5 biological replicates and error bars show standard deviation. Statistical significance was determined using one-way ANOVA followed by Dunnett’s multiple comparison test. Exact p values from left to right: a < 0.0001, 0.4946, <0.0001. Source data are provided as a Source Data file. b Gene arrangement and annotation of the hom BGC that spans 9596 bp in length. c HomA encodes a 65-amino acid precursor peptide, consisting of an N-terminal leader and a C-terminal core sequences. d The proposed post-translational modifications of the core peptide, with the modified sites shown and the modifying enzymes indicated. Image was generated in BioRender. Orange dehydrobutyrine (Dhb), green dehydroalanine (Dha), blue d-alanines, red glutamate.