Fig. 2: Imaging and Flow modules of G-Bot.
From: An integrated platform for liquid handling and cell imaging in life science applications
A Schematic illustration of the optical setup of the Imaging Module. Photographs captured by the Imaging Module regarding (B) 48-hour-old WT (wild type) AB strain zebrafish larva, (C) rabbit rectal tissue, (D) MCF-7 breast cancer cells, and (E) L929 mouse fibroblast cells. F Schematic illustration of the Flow Module. G Percentage error in the resulting volumes dispensed by the Flow Module for different target volumes of DI water. Black, blue, red and yellow: 1, 0.5, 0.25 and 0.08 μL (n = 10 repetitions for each dosing volume). H Percentage error in the seeded Jurkat cells for different target cell densities. Black, blue, red, yellow and magenta: 10 K, 20 K, 30 K, 50 K and 100 K cells/mL (n = 5 repetitions for each cell density). The box plots indicate the interquartile range (25th–75th percentile); the center line represents the median; whiskers extend to the most extreme data points within 1.5 × interquartile range; data points beyond this range are plotted individually as outliers; and squares indicate the mean values. Please note that the whiskers do not always represent the absolute minimum and maximum values when outliers are present. I Fluorescence images of MCF-7 cells remaining after the wash times of 1 and 5 for G-Bot and manual liquid handling using a pipette. In the figure, the top figures show the initial fluorescent intensities, and the bottom figures are the remaining fluorescence intensities after the PBS wash. J Remaining fluorescence intensities (FL), e.g., percentage of remaining over initial intensity, for G-Bot (blue) and manual liquid handling (red).
