Fig. 5: Phosphor-ATM phosphorylates and brings TRIM37 into the nucleus to associate with PARP1. | Nature Communications

Fig. 5: Phosphor-ATM phosphorylates and brings TRIM37 into the nucleus to associate with PARP1.

From: TRIM37–PARP1–TET1 axis maintains stemness and prevents osteoporosis by inhibiting DNMT1 alternative splicing via 5hmC regulation

Fig. 5: Phosphor-ATM phosphorylates and brings TRIM37 into the nucleus to associate with PARP1.The alternative text for this image may have been generated using AI.

a Western blotting showed that the total and phosphor-ATM (p-ATM) and TRIM37 levels increased in early MSCs. b, c Western blotting showed that ATM inhibition (b) with KU55933 (10 μM) for 2 h in early MSCs decreased the TRIM37, PARP1, and DNMT1 levels, while ATM activation (c) with resveratrol (5 μM) for 2 d in middle passage (p7-9) MSCs increased the TRIM37, PARP1 and DNMT1 levels. d, e Immunofluorescence showing increased nuclear localization of TRIM37 with (d) p-ATM or (e) PARP1 in early MSCs. f, upper panel Duolink proximity ligation assay (PLA) showed that the nuclear association of TRIM37 with p-ATM and PARP1 was increased in early MSCs compared to late MSCs. f, lower panel Quantification of the percentage of PLA nuclear-positive cells in early or late MSCs. g, upper panel Duolink PLA assay showed that ATM activation with resveratrol induced the nuclear association of TRIM37 with p-ATM and PARP1 in middle passage MSCs. g, lower panel Quantification of the percentages of PLA nuclear-positive cells in the vehicle control or resveratrol-treated MSCs. h The 293 T cells were overexpressed with wildtype (WT), T203A or T203E mutant Flag-TRIM37. Immunofluorescence with anti-Flag antibodies showed that the phosphorylation at T203 is required for the nuclear localization of TRIM37. Arrows indicate cells with nuclear translocation of specific proteins. Results are expressed as the mean ± SD of three independent experiments. (*p < 0.05; **p < 0.01; ****p < 0.0001; unpaired two-tailed t-test, or ordinary one-way ANOVA). Exact p-values are shown in the Source Data file. Scale bar: 20 μm.

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