Fig. 7: TRIM37 KO exhibited reduced bone density and disrupts bone regeneration in vivo. | Nature Communications

Fig. 7: TRIM37 KO exhibited reduced bone density and disrupts bone regeneration in vivo.

From: TRIM37–PARP1–TET1 axis maintains stemness and prevents osteoporosis by inhibiting DNMT1 alternative splicing via 5hmC regulation

Fig. 7: TRIM37 KO exhibited reduced bone density and disrupts bone regeneration in vivo.The alternative text for this image may have been generated using AI.

a Flowchart illustrating the experimental procedures. b,c, Micro-CT analysis conducted in the distal femur of mice at different stages: young (12 weeks), old (57 weeks), wild type (WT, 12 weeks), and TRIM37-deficient (TRIM37 KO, 12 weeks). Panel (b) depicts the morphological characteristics of trabecular bone in old mice, while panel (c) focuses on the comparison between WT and TRIM37 KO mice. (Lower panels) Quantification of micro-CT data on bone volume fraction (Bone volume (BV) / Total volume, %), bone mineral density (mg HA/ml), trabecular number (1/mm), and trabecular spacing (mm) (p values represent in number on top of each assessment as determined with unpaired t-test). d, e At 8 weeks of age, the medial cortex of the right femoral shafts was drilled in young versus old mice (d) and in WT versus TRIM37 KO mice (e). Three weeks later, calcein injection was performed, followed by fluorescence microscopy analysis of new bone formation another 1 week later. Representative fluorescent images from more than three independent experiments were captured using a single-photon microscope. Rectangular areas are magnified in the right. Arrow in (e) highlights the observed pinhole. Scale bar, 500 μm. n ≥ 3 biologically independent mice. Results are expressed as the mean ± SD of independent experiments. (*p < 0.05; **p < 0.01). Exact p-values are shown in the Source Data file.

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