Fig. 3: Copper-induced changes in CTR1 oligomeric states assessed by TNPD and smND.
From: Elevated intracellular copper induces CTR1 monomerization and prevents copper uptake
Probability density functions of two-nearest pairwise distance (TNPD, a) and neighbor density (ND, b) for CTR1mEos under basal (open) and Cu-stressed (filled) conditions. Copper increases TNPD and reduces ND, consistent with increased inter-subunit separation. smND-extracted oligomer populations (c) and weighted average subunits per complex (\(\bar{N}\), d) show significant Cu-dependent de-trimerization of CTR1mEos. e Trimer percentages estimated with two dissociation bounds—Model 1 (T → M + D) and Model 2 (T → 3 M)—both indicate reduced trimer content with copper. Same analyses for CTR1(M150L)mEos show no Cu-dependent change in TNPD (f), ND (g), oligomer populations (h), \(\bar{N}\) (i), nor trimer fraction (j) in rab5+ areas. Data are from three independent experiments; graphs (b, d, e, g, i, j) show mean ± SEM. n = 6 (WT-Basal), 6 (WT-Cu), 6 (M150L-Basal), 9 (M150L-Cu). d, i Two-tailed Student’s t tests; e, j ordinary one-way ANOVA with Fisher’s LSD post-hoc; exact p values indicated.
