Fig. 4: Impact of Cu on global and Rab5-specific endocytosis.
From: Elevated intracellular copper induces CTR1 monomerization and prevents copper uptake
WGA-labeled puncta area (a) and density (b) under no endocytosis control, basal conditions, and Cu-stressed conditions show no basal vs Cu difference, indicating copper does not broadly affect global endocytosis. Rab5+ puncta area (c) and density (d) in COS7 cells expressing CTR1mEos and CTR1(M150L)mEos are unchanged by copper. Density of CTR1 in Rab5+ (e) and Rab5⁻ (f) regions shows downward cell-based trends for WT with copper and directionally smaller changes for CTR1(M150L). In (a, b): n = 26 (negative control), 41 (Basal), 43 (Cu). In (c, d): n = 26 (WT-Basal), 31 (WT-Cu), 29 (M150L-Basal), 38 (M150L-Cu). In (e, f): n = 6 (WT-Basal), 6 (WT-Cu), 6 (M150L-Basal), 9 (M150L-Cu). Mean ± SEM; ordinary one-way ANOVA with Fisher’s LSD post-hoc; p values shown. Rab5⁻ denotes areas outside the Rab5⁺ segmentation of the same cell, matched in size.
