Fig. 7: Darapladib ameliorates pathological bone loss in HFD and OVX models.

A The schematic diagram of high fat diets induced bone loss model. B Micro CT images of trabecular in CD or HFD treated with or without Darapladib groups. CD Chow diets, HFD high fat diets (scale bar, 1 mm). C Analysis of bone mass parameters in the three groups. n = 6 biologically independent mice per group. D, E Staining and statistical analysis of osteoclasts presence. n = 6 biologically independent mice per group. CD group served as control, HFD group represents high-fat diet model, and HFD + Darapladib group represents treatment with Darapladib on HFD background (scale bar, normal, 200 μm. enlarged, 100 μm). F, G Micro CT images and statistical analysis of cortical thickness. n = 6 biologically independent mice per group. (scale bar, 1 mm). H The schematic diagram of the ovariectomy experiment. I, J The images and bone mass analysis of trabecular in female mice divided into sham, OVX, OVX supplemented with Darapladib groups. n = 6 biologically independent mice per group (scale bar, 1 mm). K, L The expression of osteoclasts in two staining assays and statistical analysis. n = 6 biologically independent mice per group (scale bar, normal, 200 μm, enlarged, 100 μm). M, N The diagram of cortical bone and morphological parameters of WT, OVX and Darapladib-treated OVX mice. n = 6 biologically independent mice per group (scale bar, 1 mm). Data were showed as mean ± SD. Two-way ANOVA (C, E, G, J, L, N) was utilized for multiple comparisons test. Significance: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, ns non-significance.