Fig. 6: ARRB2 mediates cGAL53 effects in intestinal colitis. | Nature Communications

Fig. 6: ARRB2 mediates cGAL53 effects in intestinal colitis.

From: A novel long-galanin peptide from non-mammalian vertebrates mitigates the inflammatory response in IBD models via the biased GALR2/β-arrestin2 pathway

Fig. 6

a AAV8-Vil-cre and AAV8-null were used to infect Arrbfl/fl mice (7–8 weeks) to generate intestinal epithelial-specific Arrb2 KO mice (ArrbΔVil) and control mice (Arrbfl/fl) for the 2% DSS-induced colitis model. Silhouette from PhyloPic.org. Change of body weight (b) and DAI (c) in Arrb2 cKO mice and control mice throughout the experimental period (n = 5/group). d Representative images of colon and quantification of colon length in Arrb2 cKO mice and control mice (n = 5/group). e Representative images of H&E staining. Scale bar = 200 μm. f ELISA was used to quantify serum IL-6 and IL-1β levels in each group of Arrb2 cKO mice (n = 5/group). g, h Quantitative evaluation of inflammation cytokines (Il6, Tnf, Il1b, and Tgfb1) and tight junction protein (Tjp1 and Ocln) expression in the colon of every group in Arrb2 cKO mice and control mice (n = 5/group). Statistical significance was determined using two-way ANOVA (for b, c) or one-way ANOVA followed by a multiple comparisons test (for d, e, f, h). Data are presented as mean ± SD. Statistically significant differences between groups are indicated with asterisks: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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