Fig. 5: Transcriptome analysis reveals the genetic background of abundance of hydroxy fatty acids in three Physaria species.
From: Chromosome-level assembly and analysis of three hydroxy fatty acid-producing Physaria species
a Contents of different types of fatty acids in seed oils, among which lesquerolic acid (C20:1-OH) accounts for 80.78%, 84.56% and 51.19% in stored triacylglycerol in P. lindheimeri, P. pallida and P. fendleri, respectively. Error bars represent mean values ± SD. For each type of fatty acid, n = 6 biological replicates. b The simplified diagram of the lipid biosynthesis pathway in three Physaria species. The heatmaps represents the expression of each gene and key transcription factors in seeds at different developmental stages (EDS: early developing seed, MDS: middle developing seed, LDS: late developing seed), as well as leaf and stem. The red color of heatmap indicates high gene expression, while blue indicate low expression. ACCase acetyl-CoA carboxylase, FAS fatty acid synthase, KAS Ⅱ β-ketoacyl-ACP synthase Ⅱ, SAD stearoyl-acyl carrier protein desaturase, FATA fatty acid thioesterase A, FATB fatty acid thioesterase B, LACS long-chain acyl-CoA synthetase, LPCAT lysophosphatidyl-choline acyltransferase, FAD2 fatty acid desaturase 2, FAD3 fatty acid desaturase 3, FAH12 oleate 12-hydroxylase, KCS3 3-ketoacyl-CoA synthase 3, PLC phospholipase C, PDCT phosphatidylcholine: diacylglycerol cholinephosphotransferase, CPT CDP choline: diacylglycerol cholinephosphotransferase, PDAT: phospholipid diacylglycerol acyltransferase, GPAT glycerol-3-phosphate acyltransferase, LPAT lysophosphatidic acid acyltransferase, PAP phosphatidic acid phosphatase, DGAT diacylglycerol acyltransferase, ABI3 abscisic acid insensitive 3, WRI1 wrinkled 1, FUS3 fusca 3, LEC1 leafy cotyledon 1, LEC2 leafy cotyledon 2. c Phylogenetic trees and gene expression profiles of FAH12 and FAD2, KCS3 and FAE1 genes in R. communis, A. thaliana, P. lindheimeri, P. pallida and P. fendleri. A. thaliana FAD2 (NP_001319529.1), R. communis FAD2 (QCO64167.1), R. communis FAH12 (ACD39348.1), P. fendleri FAD2 (AFP21683.1), P. fendleri FAH12 (ACQ42234.1), A. thaliana KCS3 (NP_001322003.1), A. thaliana FAE1 (NP_195178.1), P. fendleri KCS3 (AAK62348.1) genes were obtained from NCBI database (https://www.ncbi.nlm.nih.gov/). Source data are provided as a Source Data file.
