Fig. 1: Single-cell landscape analysis of CCI samples based on single-cell sequencing.
A t-SNE clustering map of annotated cell types after batch effect removal, extracted from single cells in CCI model mice (7D and 14D) and their contralateral standard dorsal spinal tissue samples; B Intercellular ligand–receptor interaction networks were predicted using the CellPhoneDB database. Node size represents gene expression intensity, and edge thickness indicates interaction frequency. The figure illustrates interactions between microglia and astrocytes; C Volcano plot of DEGs in various subgroups. Yellow dots represent downregulated genes, and purple dots indicate upregulated genes; D Heatmap of GO-BP enrichment analysis for each subgroup. The left line chart represents gene expression abundance, the middle heatmap shows gene cluster expression, and the right side displays the GO analysis BP enrichment results; E t-SNE clustering map for microglial subpopulations; F Distribution of the anti-inflammatory polarization-related gene Tgfb1 in microglia subgroups; G Clustering distribution of microglia subgroups on the developmental tree, with different colors representing different cell subgroups; H t-SNE clustering map for astrocytes subgroups; I Distribution of the neuroprotective astrocyte-related gene S100A10 in astrocytes subgroups; J Clustering distribution of astrocyte subgroups on the developmental tree, with different colors representing different cell subgroups. RNA-seq data in this figure derive from GEO: GSE20876629.
