Fig. 2: The E3 ubiquitin ligase FBXL18 promotes IL-6 production in RABV-infected astrocytes. | Nature Communications

Fig. 2: The E3 ubiquitin ligase FBXL18 promotes IL-6 production in RABV-infected astrocytes.

From: The E3 ubiquitin ligase FBXL18 stabilizes BST2 to promote inflammation in RABV-infected astrocytes

Fig. 2: The E3 ubiquitin ligase FBXL18 promotes IL-6 production in RABV-infected astrocytes.The alternative text for this image may have been generated using AI.

A MA-C cells transfected with specific siRNAs were infected with CVS-11 for 24 h, the level of IL-6 was analyzed by ELISA. B, C MA-C cells were transfected with the siRNA against FBXL18, plasmid for HA-FBXL18 expression, or negative control and then infected with CVS-11 for 24 h. The mRNA levels of FBXL18, IL-6, CXCL-10 were analyzed by RT-qPCR; FBXL18, viral N/P protein were analyzed using IB with the corresponding antibodies. D–F The MA-C cells and N2a cells were infected with CVS-11 for indicated time, the FBXL18 protein level was analyzed using IB (D), and the mRNA level was analyzed using RT-qPCR (F). The relative expression level of FBXL18 normalizing to GAPDH or β-actin using Image J (E). Data show in results (A–C and E, F) were mean ± s.e. from three biologically independent experiment each done in triplicate. Unpaired two-tailed Student’s t-test were performed. Source data are provided as a Source Data file.

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