Fig. 5: AGPAT2 and DRP1 function together in ER tubulation.
From: AGPAT2 acts at the crossroads of lipid biosynthesis and DRP1-mediated ER morphogenesis
a WT, RTN4A-KO, and DRP1-KO cells were transfected with AGPAT2-FLAG. The ER was visualized using mCherry-SEC61β. b Quantification of ER morphology. Mean ± SD (n = 3 experiments). c AGPAT2-KO and RTN4A-KO MEFs were transfected with DRP1. The ER was visualized using GFP-SEC61β. d Quantification of ER morphology. Mean ± SD (n = 3 experiments). e WT and AGPAT2-KO MEFs were transfected with RTN4A-GFP. The ER was visualized using mCherry-SEC61β. f Quantification of ER morphology. Mean ± SD (n = 3 experiments). Statistical analysis was performed using one-way ANOVA with post-hoc Tukey (b, f) and two-tailed Student’s t-test (d). Source data are provided as a Source Data file.
