Fig. 4: THBS2 loss in CAFs reshapes the immune landscape of fibrotic TMEs.

a,b UMAP of single-cell RNA sequencing (scRNA-seq) data of orthotopic MTO tumors in WT or Thbs2^-/- mice. all tumor cells colored by host mouse genotypes (a) and major cellular components (b). c Pie charts showing the proportions of indicated cell types in each genotype. d, e UMAP of immune cells colored by cell types (d) and proportion of each cell type (e). f UMAP of T and natural killer cells (T/NK cells) colored by cell types. g Proportion of T/NK cell types. h, i UMAP of CD8⁺ T cells colored by cell subtypes (h) and cell counts of each subtype (i). j, k Violin plots of CD8⁺ T cells (j) and monocytes/macrophages (Mono/Macs) (k) for indicated gene signatures. l Immunofluorescence staining of indicated proteins in orthotopic tumors. Scale bars, 100 μm. m Violin plots of Mono/Mac for indicated gene signatures. n UMAP of Mono/Mac colored by cell subtypes. o–q Proportion (o) and violin plots for indicated gene signatures and gene expression (p, q) of Mono/Mac subtypes. r–t UMAP of DCs colored by DC subtypes (r). UMAP feature plots of DCs in WT and Thbs2^-/- mice for indicated gene expression (s). t Violin plots of cDCs for indicated genes signatures. u, v UMAP of all tumor cells (u) and immune cells (v) colored by cell types (left) and feature plots for Cxcl10 (right). w UMAP of T/NK cells colored by cell types (left) and feature plots for Cxcr3 (right). x Schematic representation of models for CD8⁺ T cell recruitment regulated by THBS2⁺ CAF. Wilcoxon rank sum test, two-sided (j,k,m,p,q,t). Source data are provided as a Source Data file.