Fig. 7: Inhibition of CXCL9/10-CXCR3 axis suppresses CD8+ T cell recruitment in Thbs2-/- mice. | Nature Communications

Fig. 7: Inhibition of CXCL9/10-CXCR3 axis suppresses CD8+ T cell recruitment in Thbs2-/- mice.

From: Targeting fibroblast derived thrombospondin 2 disrupts an immune-exclusionary environment at the tumor front in colorectal cancer

Fig. 7

a–e Combined blockage of CXCL9 (αCXCL9 ab) and CXCL10 (αCXCL10 ab) in MTO-bearing WT or Thbs2-/- mice (n: WT control = 8, WT αCXCL9 ab/CXCL10 ab = 9, Thbs2-/- control = 6, Thbs2-/- αCXCL9 ab/CXCL10 ab = 9). Schematic representation (a), macroscopic images (b), and volumes and weights (c) of tumors. Immunofluorescence for CD8 (d) and its quantification (e). Bottom images show magnified views of the yellow dashed boxes in the top panels (d). f–o Anti-CXCR3 antibody (αCXCR3 ab) treatment in MTO-bearing WT or Thbs2-/- mice (n: WT control = 10, WT αCXCR3 ab = 10, Thbs2-/- control = 7, Thbs2-/- αCXCR3 ab = 8). Schematic representation (f) macroscopic images (g) and volumes and weights (h) of tumors. i Representative IMC images of orthotopic tumors. T: tumor, NT: non-tumor. j, k CD8+ T cell infiltration analysis from (f). Histograms (j) and scatter plots (k) show distances of CD8+ T cells from tumor borders. l–o, Proximity analyses between CD8+ T cells and F4/80+ macrophages or CD11c+ DCs in (f). Histograms (l) and scatter plots (m) show the nearest distances between CD8+ T cells and CD11c+ DCs. Histograms (n) and scatter plots (o) show the nearest distances between CD8+ T cells and F4/80+ macrophages. Red dashed lines indicate tumor regions in (b,g). White lines denote tumor borders in (i). Scale bars, 1 mm (b, g), 500 μm (d, top), 50 μm (d, bottom), 100 μm (i). Fisher’s LSD test (c,e,h), Mann-Whitney U-test, two-sided (k,m,o). Mean ± SEM. Source data are provided as a Source Data file.

Back to article page