Fig. 1: Decreased locomotion, associative learning and sensory discrimination in Akap11 mutant mice. | Nature Communications

Fig. 1: Decreased locomotion, associative learning and sensory discrimination in Akap11 mutant mice.

From: Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder

Fig. 1

a Heatmap of average activity of each genotype across 24 h in a homecage locomotor activity monitoring system. Each row is 24 h, and 60 rows representing 60 consecutive days are shown. Yellow and black bars indicate lights-on and lights-off periods respectively. ZT (Zeitgeber Time) refers to the number of hours past the lights-on time in light-dark scheduled conditions. b Averaged locomotor activity of each WT and Akap11 mutant mice, across 60 days of the assay. c Locomotor activity averaged across 24 h, aligned according to animals’ postnatal ages. d Running wheel usage during a 1 week test period. e Syllable usage of WT and Akap11 mutant mice (n = 11 mice for WT, n = 11 mice for Akap11+/-, and n = 10 mice for Akap11-/-), sorted by usage difference between WT and Akap11-/-. Color codes on the x-axis represent annotated behaviors. * Represents represent syllables with FDR < 0.1. Data are represented as mean ± 95% confidence interval. f Relative syllable usage of WT and Akap11 mutant mice sorted by velocity. g Syllable transition matrix showing the upregulated (red lines) and downregulated (blue lines) transition probability between Akap11+/- and WT (left) or between Akap11-/- and WT (right). h Average usage of significantly downregulated rearing syllables (top), grooming syllables (middle), and significantly upregulated pausing syllables (bottom) between Akap11 mutants and WT (n = 11 mice for WT, n = 11 mice for Akap11+/-, and n = 10 mice for Akap11-/-). The box plots display the median (white dot) and interquartile range (25–75th percentile); the whiskers represent upper and lower adjacent values; the outer bounds represent the data distribution. i Schematic diagram of the associative learning and sensory discrimination task. j During fear conditioning (Day 1), freezing time (%) to CS+ (left) and CS- (middle) by WT and Akap11 mutant mice. Bar graph (right) represents the freezing time (%) during trials 6–10. k During fear recall (day 2), freezing time (%) and to CS+ and CS- by WT and Akap11 mutant mice (left). Discrimination index (right) is defined as: (CS+ freeze - CS- freeze) / (CS+ freeze + CS- freeze). b,c,d,j,k data are represented as mean +/- SEM, and bar graphs as mean + SEM. b,d,h One-way ANOVA with Tukey’s post hoc test. See Supplementary Data 1 for statistics. j,k Repeated measures two-way ANOVA with Tukey’s post hoc test for CS+ and CS- freezing time (%), and one-way ANOVA with Bonferroni post hoc test for discrimination index. See Supplementary Data 1 for statistics.

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