Fig. 6: WEE1i and PKMYT1i synergistically induce cytotoxicity without WEE1i-induced ISR activation.
From: WEE1 inhibitors trigger GCN2-mediated activation of the integrated stress response
a Relative ATF4-mScarlet MFI in RPE1 TP53KO ATF4-mScarlet reporter cells after treatment with the indicated doses of Debio 0123. Data represent mean ± SD (n = 3). b Cell viability in parental RPE1 TP53KO PACKO, GCN2KO #1 and GCN2KO #2 cells after treatment with Debio 0123 and RP-6306 at the indicated doses (n = 5). c ZIP synergy scores from cell survival matrices shown in (b, d). Data represent mean ± SD (n = 5). d Synergy plots with ZIP synergy scores of heatmaps shown in (b). e Relative ATF4-mScarlet MFI in RPE1 TP53KO ATF4-mScarlet reporter cells treated with the indicated doses of AZD1775, Debio 0123 and RP-6306. Data represent mean ± SD (n = 4). Statistical analysis (a, c, e) was performed using paired t-tests (two-sided), with p ≤ 0.05 considered significant. All replicates are biological replicates unless indicated otherwise. Source data are provided as a Source data file.
