Fig. 7: Demonstration of 35S-GTPγS binding and release in membrane preparations from dorsal horn of human spinal cord.
From: Characterization of the GTPγS release function of a G protein-coupled receptor
A Dorsal horn was dissected from a previously frozen L4 section of human spinal cord; the “pulse” loading of GTPγS was performed in the presence of sodium chloride as described in Fig. 1. B The loading of 35S-GTPγS in the pulse reaction produces a 15 ± 2% increase in the presence of 100 nM DAMGO (**p < 0.01 paired two-tailed Student’s t-test, n = 3 individual spinal cords). C 35S-GTPγS binding and D. release are demonstrated using DAMGO and loperamide in the same samples presented in B (mean with s.e.m. plotted). The pEC50 (M) and Emax (% stimulation over baseline) are presented in the table inset with 95% CI. BioRender® was used to create the schematic, with license.
