Fig. 3: DHC to DHEA conversion does not require CYP17A1.

A Intracellular DHEA, testosterone, and DHT levels in charcoal-stripped serum starved or serum-free starved C4-2 cells. Cells were starved, treated with 100 nM DHC with or without CYP17A1 inhibitors, and harvested after 5 days. From n = 3 biological intra-assay replicate samples. Shown are pathway consequences of galeterone blockade of 3βHSD1. B Structure of deuterium-labeled (20 R)−17α,20-dihydroxycholesterol (DHC). C Intracellular deuterium-labeled androgens or DHEA levels in ethanol-, abiraterone-, or ketoconazole-treated C4-2 cells. Cells were starved, treated with 100 nM deuterium-labeled DHC with or without CYP17A1 inhibitors, and harvested after 5 days. From n = 3 biological intra-assay replicate samples. All measurements were done by LC-MS/MS and are presented as mean +/− SD. Shown are pathway consequences of abiraterone blockade of 3βHSD1 and ketoconazole blockade of an unknown cytochrome enzyme. Source data are provided as a Source Data file.