Fig. 4: The existence of methane-oxidizing gut microbiota can be validated in multiple cohorts.

A The establishment of Methane-oxidation genes (MOGs) and Non-MOGs databases. B Geographic distribution of samples with the fecal samples detected MOG. Each point indicates one sampling location. To visualization, overlapping points randomly increase latitude and longitude. C The correlation between the sequencing depth and detected MOG read count (mapped read). D The difference of the sequencing depth between detected MOG samples and Not-detected MOG samples (Detected n = 504, Not-detected n = 46). E The different gut MOB relative abundance among different cohorts (Health n = 500, Obesity n = 475, Constipation n = 232). F The correlation between the BMI and relative abundance of gut MOB. G The correlation between the fecal consistency and relative abundance of gut MOB. H ROC analysis of gut MOB relative abundance in cohorts. Solid lines represent ordinary least-squares linear regressions, and the error bands indicate the 95% confidence intervals (C, F, H). Statistical analysis was performed with two-tailed Spearman’s correlation analysis (C, F, G) and two-tailed Mann–Whitney–Wilcoxon test with Benjamini/Hochberg correction (D, E). Individual data points are independent biological replicates unless otherwise stated. Source data are provided as a Source Data file.