Fig. 6: Visual function analysis of MT-ND4 mutant mice using OKN and ERG.
From: In vivo mitochondrial base editing restores genotype and visual function in a mouse model of LHON
a m.G11185A editing efficiency in 26-week-old mice with visual phenotypes, with independent-samples for each group (WT, n = 11, PBS injected ND4 mutant, n = 16, AAV injected ND4 mutant, n = 5). b Schematic illustration of the optokinetic nystagmus (OKN). Created in BioRender. Kim, K. (2025) https://BioRender.com/vhbzqbr. c Visual acuity measured in WT (n = 11), PBS-injected (n = 16), and sTALED-V28R AAVs (n = 5) injected MT-ND4 mutant mice. The exact p-values were 0.011 with PBS-injected and sTALED-V28R AAVs injected, 0.19 on sTALED-V28R AAVs injected MT-ND4 mutant mice compared with WT mice. Error bars indicate standard error of the mean (s.e.m.) for biologically independent mice. (n.s. not significant, using Student’s two-tailed t-test). d Schematic illustration of electroretinography (ERG). Created in BioRender. Kim, K. (2025) https://BioRender.com/lpzv9ih. e a-wave amplitude measured in WT (n = 11), PBS-injected (n = 16), and sTALED-V28R AAVs (n = 5) injected MT-ND4 mutant mice, showing no significant difference between the three groups. Error bars indicate standard error of the mean (s.e.m.) for biologically independent mice. (n.s. not significant, using Student’s two-tailed t-test). f b-wave amplitude measured in WT (n = 11), PBS-injected (n = 16), and sTALED-V28R AAVs (n = 5) injected MT-ND4 mutant mice, showing no significant difference between the three groups. Error bars indicate standard error of the mean (s.e.m.) for biologically independent mice. (n.s. not significant, using Student’s two-tailed t-test). g Photopic negative response (PhNR) measured in WT (n = 11), PBS-injected (n = 16), and sTALED-V28R AAVs (n = 5) injected MT-ND4 mutant mice. The exact p-values were 0.025 with PBS-injected and sTALED-V28R AAVs injected, 0.64 on sTALED-V28R AAVs injected MT-ND4 mutant mice compared with WT mice. Error bars indicate standard error of the mean (s.e.m.) for biologically independent mice. (n.s. not significant, *p < 0.05 using Student’s two-tailed t-test). h Recorded electronic waves used in ERG analysis of WT, PBS-injected, and sTALED-V28R AAVs injected MT-ND4 mutant mice. Individual mouse waves are shown in gray, and the average wave for each group is shown as a bold line.
