Fig. 1: Single nuclei combined RNA and ATAC analysis of E15.5 and 18.5 mouse lungs. | Nature Communications

Fig. 1: Single nuclei combined RNA and ATAC analysis of E15.5 and 18.5 mouse lungs.

From: Epithelial-mesenchymal cell competition coordinates fate transitions across tissue compartments during lung development and fibrosis

Fig. 1

A Graph-based clustering and cell-type or sample annotation of integrated datasets from E15 and E18 ctrl and mutant lungs based on enriched gene expression and chromatin accessibility profiles by using ArchR. AF1ss (AF1 population in Snai1/2 inactivated mesenchyme), AF1yt (AF1 population in Yap/Taz inactivated mesenchyme). Subsetting and clustering of B mesenchyme and C epithelium. Graph-based clustering and cell-type annotation on individual samples of D mesenchyme and E epithelium. F Tead motif accessibility and representative MAGIC imputed gene expression for main mesenchymal and epithelial clusters. G Heatmap of differential transcription factor motif accessibility showing which regulatory factors are predicted to most active in each cell type. AF1ss (AF1 population in Snai1/2 inactivated mesenchyme) contains enrichment for myogenic differentiation and Tead transcription factors (green), AF1 is enriched for adipogenic factors (yellow), AF1yt (AF1 population in Yap/Taz inactivated mesenchyme) lacks Tead binding activity.

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