Fig. 3: Loss of Yap/Taz in AF1s promotes bronchiolization after bleomycin injury.
A Mice were placed on tamoxifen chow for 3 weeks, after a 3 week washout, lungs were injured with bleomycin and dissected 6 weeks later. Created in BioRender. Warren, R. (2025) https://BioRender.com/buq1mhz. B–D Immunostaining for GFP and Krt5 on B control (n = 7), C Plin2CreERT2;Yap1f/f;Wwtr1f/f;mTmG (n = 12) lungs 6 weeks after bleomycin injury. Scale bar 500 µm. D Nanostring nCounter RNA analysis for Krt5 on control (n = 42), Plin2CreERT2;Yap1f/f;Wwtr1f/f;mTmG (n = 13, p < 0.0001) and Plin2CreERT2;Stk3/4f/f (n = 11, p < 0.0001). E Image quantification of GFP in images represented in (B, C) (p = 0.004). Immunostaining for Krt8 in F control (n = 4), G Plin2CreERT2;Yap1f/f;Wwtr1f/f (n = 3, p = 0.03), H Plin2CreERT2;Stk3/4f/f (n = 6, p = 0.04), and I Scube2CreERT2;Yap1f/f;Wwtr1f/f (n = 5, p = 0.04) lungs 6 weeks after bleomycin injury. Scale bar 500 µm. J Image quantification of Krt8 in images represented in (F–I). K Hydroxyproline analysis on bleomycin injured Scube2CreERT2;mTmG controls (n = 43) and Scube2CreERT2;Yap1f/f;Wwtr1f/f;mTmG (n = 30, p = 0.04). L, M TUNEL staining and quantification on bleomycin injured control Plin2CreERT2;mTmG (n = 3) and Plin2CreERT2;Yap1f/f;Wwtr1f/f;mTmG (n = 3, p = 0.001) lungs. Data are presented as mean values +/−SEM. Two tailed unpaired T-test was used to determine significance. F test was used to determine equal variances and unreported F values indicate equal variance. *p < 0.05, **p < 0.01, ****p < 0.0001.
