Fig. 6: Loss of Myc in AF1s promotes bronchiolization after bleomycin injury. | Nature Communications

Fig. 6: Loss of Myc in AF1s promotes bronchiolization after bleomycin injury.

From: Epithelial-mesenchymal cell competition coordinates fate transitions across tissue compartments during lung development and fibrosis

Fig. 6

A Mice were placed on tamoxifen chow for 3 weeks, after a 3 week washout, lungs were injured with bleomycin and dissected 6 weeks later. Created in BioRender. Warren, R. (2025) https://BioRender.com/buq1mhz. B Immunostaining for Krt8 (p = 0.03) and Krt5 (p = 0.005) on control (n = 6) and Plin2CreERT2;Mycf/f;mTmG (n = 11) lungs 6 weeks after bleomycin injury. Scale bar 200 µm. C Hydroxyproline analysis on bleomycin injured Plin2CreERT2;mTmG (n = 23) and Plin2CreERT2;Mycf/f;mTmG (n = 34) lungs (p = 0.009). D Image quantification of Krt8 and Krt5 in images represented in (B). E qPCR analysis on RNA from bleomycin injured Cre- controls (n = 19) and Plin2CreERT2;Mycf/f;mTmG (n = 22) lungs for Krt5 (p = 0.03) and Trp63 (p = 0.03) genes. Data are presented as mean values +/− SEM. Two tailed unpaired T-test was used to determine significance. F test was used to determine equal variances and unreported F values indicate equal variance. *p < 0.05, **p < 0.01.

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