Fig. 7: Snail1/2 are required for myofibroblast dedifferentiation into AF1s during the resolution of pulmonary fibrosis. | Nature Communications

Fig. 7: Snail1/2 are required for myofibroblast dedifferentiation into AF1s during the resolution of pulmonary fibrosis.

From: Epithelial-mesenchymal cell competition coordinates fate transitions across tissue compartments during lung development and fibrosis

Fig. 7

A Mice were injured with bleomycin and 2 weeks after injury, they were placed on tamoxifen chow until dissection at 6 weeks after injury. Created in BioRender. Warren, R. (2025) https://BioRender.com/xo6zwap. B Mesenchymal cell clusters from scRNAseq on non injured control and bleomycin injured control and Acta2CreERT2;Snai1/2f/f lungs tamoxifen induced 2 weeks after bleomycin injury. C Fibrosis promoting pathway activities are upregulated in AF1-SS (AF1 population in Snai1/2 inactivated mesenchyme). D Hydroxyproline analysis of soluble collagen on control (n = 30) and Acta2CreERT2;Snai1/2f/f (n = 7, p = 0.0001). E Co-immunostaining for GFP and Acta2 on bleomycin injured control (n = 3) and Acta2CreERT2;Snai1/2f/f (n = 7, p = 0.02) lungs tamoxifen induced 2 weeks after bleomycin injury. Scale bar 100 µm. F Quantification of GFP in images represented in (E). Data are presented as mean values +/− SEM. Two tailed unpaired T-test was used to determine significance. F test was used to determine equal variances and unreported F values indicate equal variance. *p < 0.05, ***p < 0.001.

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