Fig. 1: Analysis of aptamer binding to RAD51. | Nature Communications

Fig. 1: Analysis of aptamer binding to RAD51.

From: Computationally-designed aptamers targeting RAD51-BRCA2 interaction impair homologous recombination and induce synthetic lethality

Fig. 1

A BLI analysis illustrating the binding kinetics of the first 6 aptamers (Apt1 to Apt6) and a negative control (rcApt) with RAD51 protein at 25 °C. Each curve represents the interaction profile of an individual aptamer, from which their respective binding affinities towards RAD51 can be extrapolated. Spheres: experimental points; line: curve fitting. The curve relative to rcApt is reported as raw data, since no binding response was registered. Three replicates were performed, and the mean and standard deviation were calculated for each point within the binding curves. Below each curve, the dissociation constant (Kd) is indicated. (ND = not detectable). B A scatter plot showing the correlation between experimentally determined Kds of the aptamers with RAD51 and their affinities predicted by the catRAPID algorithm for Region 2. Source data are provided as a Source Data file.

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