Fig. 4: FLIM analysis of Apt1 affecting RAD51 and BRC4 interaction. | Nature Communications

Fig. 4: FLIM analysis of Apt1 affecting RAD51 and BRC4 interaction.

From: Computationally-designed aptamers targeting RAD51-BRCA2 interaction impair homologous recombination and induce synthetic lethality

Fig. 4: FLIM analysis of Apt1 affecting RAD51 and BRC4 interaction.

Phasor plot from FLIM analysis showing sine component (S) and cosine component (G) on the axes, for the fluorophore Texas Red conjugated to the aptamers Apt1 and rcApt. Points A–F correspond to different experimental conditions. A Apt1 alone. B Apt1 after incubation with RAD51. C Apt1 with RAD51 followed by the addition of BRC4. D rcApt alone. E rcApt after incubation with RAD51. F rcApt with RAD51 followed by the addition of BRC4. D–F Results are perfectly overlapping. Source data are provided as a Source Data file.

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