Fig. 5: Effect of increasing concentrations of Apt1 on RAD51 and BRCA2 nuclear localization in response to DNA damage. | Nature Communications

Fig. 5: Effect of increasing concentrations of Apt1 on RAD51 and BRCA2 nuclear localization in response to DNA damage.

From: Computationally-designed aptamers targeting RAD51-BRCA2 interaction impair homologous recombination and induce synthetic lethality

Fig. 5

A High-resolution immunofluorescence images of a single BxPC-3 cell untreated or treated with increasing concentrations of Apt1 in combination with cisplatin. Nuclei are stained with DAPI (blue); RAD51 is shown in green; BRCA2 in red. Scale bar: 10 μm. B Bar graphs illustrating the image-based quantification of a cell population nuclear fluorescence, showing RAD51 (orange) and BRCA2 (blue) under DNA damage conditions and increasing concentrations of Apt1. Experiments were performed in biological triplicates, and data were collected from approximately 500 cells per condition. Source data are provided as a Source Data file.

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