Table 2 Fluorescence lifetime imaging

From: Computationally-designed aptamers targeting RAD51-BRCA2 interaction impair homologous recombination and induce synthetic lethality

Point

Condition

τfast (ns)

τslow (ns)

G (°)

S (°)

Fast component ratio

Slow component ratio

A

Apt 1

1.11 ± 0.2

5.01 ± 0.1

0.322

0.368

0.29

0.71

B

Apt1 + RAD51

1.34 ± 0.1

5.32 ± 0.2

0.226

0.355

0.18

0.82

C

Apt1 + RAD51 + BRC4

1.22 ± 0.3

5.09 ± 0.1

0.304

0.367

0.29

0.71

D

rcApt

–

5.1 ± 0.1

0.128

0.329

–

1.0

E

rcApt + RAD51

–

5.1 ± 0.1

0.128

0.329

–

1.0

F

rcApt + RAD51 + BRC4

–

5.1 ± 0.1

0.128

0.329

–

1.0

  1. Faster (τfast) and slower (τslow) components of the fluorescence decay lifetime, sine component (S), cosine component (G), and values for fast and slow components ratio obtained from fluorescence lifetime imaging microscopy (FLIM). These measurements refer to the fluorophore Texas Red conjugated to Apt1 and rcApt aptamers, detailing variations upon the addition of RAD51 and BRC4. The observed change in t values for Apt1 in the presence of RAD51 substantiates a binding between them. On the other hand, the t values for the control molecule, rcApt, remain unchanged, confirming its lack of interaction with RAD51.
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