Fig. 5: Mechanisms driving AtMCM10 phase separation. | Nature Communications

Fig. 5: Mechanisms driving AtMCM10 phase separation.

From: AtMCM10 facilitates SDSA-mediated intermolecular homologous recombination repair via liquid-liquid phase separation in DNA damage response

Fig. 5

a Phase separation assays of truncated AtMCM10s in vitro. AtMCM10 was truncated into IDR1, IDR2, ΔIDR1, ΔIDR2, and ΔIDR1/2 fragments. Bars = 5  μm. b The subcellular localizations of AtMCM10-ΔIDR1, ΔIDR2 and ΔIDR1/2 in Nicotiana benthamiana. The mNeon-MCM10-ΔIDR1, ΔIDR2 and ΔIDR1/2 were infiltrated into Nicotiana benthamiana leaves for transient expression. The transfected leaves were treated with 150 mM NaCl for 24 h. Bar = 5 (top) or 25 (bottom) μm. c Statistical analyses of AtMCM10 puncta upon NaCl treatment in (b). Data were presented as median with interquartile range, whiskers extending to 1.5× the interquartile range, and individual data points plotted as dots (n = 25 nuclei), and lowercase letters indicate significant differences by one-way ANOVA followed by Tukey’s HSD test (p ≤ 0.05). d Conditions for driving AtMCM10 phase separation in vitro. The ssDNA and dsDNA were labeled with Cy5.5 at 5’ end, and reactions were performed in annealing buffer. Bars = 5 μm. The fluorescent signals in (a, b, d) were observed using a Leica STELLARIS5 microscope.

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