Fig. 3: GTPBP1•aa-tRNA•GDPCP adopts different conformations on the ribosome.
From: Structural mechanism of mRNA decoding by mammalian GTPase GTPBP1
A Comparison of GTPBP1 positions in Structures IIa-d and III aligned by 28S rRNA. B Positions of catalytic His256 relative to the A4607 of the SRL in Structures IIa-d (pale colors) and III (bright colors). C Comparison of tRNA elbow positions in Structures IIa-d (pale colors) and III (bright colors). D Pie chart representing proportions of ribosome particles corresponding to Structures IIa-d and Structure III. Source data are provided as a Source data file. E Worm diagram representing maximal RMSD in the ensemble of GTPBP1•aa-tRNA Structures IIa-d and III aligned via GTPBP1. F Rearrangements of the small subunit of the ribosomes with the vacant A site (Structures Ia-c, left panel) upon binding of GTPBP1•aa-tRNA•GDPCP (Structure IIa, middle panel) and docking of GTPBP1 into the SRL (Structure III, right panel). Cylinders’ lengths and colors are proportional to maximal root-mean-square distances within the ensemble of structures specified on the top of each panel. G Close-up view of the decoding center in Structures IIa-d and III. H Comparison of Phe-tRNAPhe positions in Structures IIa-d through Structure III to Structure V (accommodated tRNA), aligned by 28S RNA.
