Fig. 1: Species-specific susceptibility to GS-inhibiting herbicide.
A–C Response to 300 µM of acetochlor (Ace), glyphosate (Gly), metsulfuron-methyl (MetSul), or phosphinothricin (PPT) for four days (A. thaliana) and five days (M. polymorpha). Colors in the heatmap represent mean chlorophyll index (B) and plant size (C). Asterisks indicate p-value < 0.05 when compared against the no-herbicide control (Ctrl), as determined by one-way ANOVA and Dunnett’s post-hoc test. n = 3. Raw data is provided in Fig. S1 and source data. D–F Growth of A. thaliana and G–I M. polymorpha in response to three different GS inhibitors. Representative images of A. thaliana (G) grown on a concentration gradient of PPT (●), bialaphos (■), or MetSox (▲) for eight days (A. thaliana) and five days (M. polymorpha). Estimated chlorophyll content (E, H) and plant size (F, I) were quantitated from images using ImageJ. Dose response curve was estimated using a sigmoid function, and the IC50 values are listed in text of the same color. Each data point represents a single biological replicate. n = 6 (A. thaliana) and 4 (M. polymorpha). Source data are provided as a Source Data file.
