Fig. 3: Non-target site mechanisms underlying PPT resistance in M. polymorpha. | Nature Communications

Fig. 3: Non-target site mechanisms underlying PPT resistance in M. polymorpha.

From: Cationic amino acid transporters (CAT) enhance accumulation and susceptibility to the systemic herbicide L-phosphinothricin

Fig. 3

A Dose response curve of GS for PPT. Crude protein extracts from A. thaliana and M. polymorpha were treated with increasing concentrations of PPT. Reactions were stopped and the absorbance of y-glutamyl hydroxamate used as a measure of GS activity. Data points and error bars reflect mean and standard deviation, respectively. n = 3 biological replicates. Slopes of inhibited activity were analysed by GraphPad7 software to obtain the log10(IC50) values, which are listed in text of the same color with the upper and lower 95% confidence intervals in brackets. B, C Accumulation of PPT (B) and MetSox (C) in A. thaliana and M. polymorpha tissues. A. thaliana was treated for four days. M. polymorpha was treated with bialaphos and MetSox for five days, and PPT for eight days. Each data point represents a single biological replicate. n = 4 for all treatments except MetSox-treated M. polymorpha and PPT-treated A. thaliana, where n = 3. Bars and error bars show the mean and standard deviation. Bialaphos was not detected in any samples. Source data are provided as a Source Data file.

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