Fig. 4: Identification of AtCATs as mediators of PPT accumulation.
A Heatmap of log2-fold change in A. thaliana and M. polymorpha AAP and CAT family genes. Asterisks indicate adjusted p-value < 0.05 (*) or 0.01 (**) as determined by Wald’s test with Benjamini-Hochberg adjustment, while gray cells indicate no gene expression. Genes highlighted in red were selected for overexpression in M. polymorpha. Gene accessions are provided in Table S2. B, C Growth of Tak-1 and transporter-overexpressing M. polymorpha lines on a concentration gradient of PPT. Plant size (B) was quantitated from images using ImageJ. Dose response curve was estimated using a sigmoid function, and the IC50 values are listed in text of the same color. Each data point represents a single biological replicate. n = 3. Only the phenotypes of selected lines (C) are shown. D Accumulation of PPT in Tak-1 and transporter-overexpressing M. polymorpha after 5 days of 100 µM PPT treatment. Data points, bars, and error bars reflect individual replicates, mean, and standard deviation, respectively. n = 3 biological replicates. The subplot shows time-dependent uptake in Tak-1 and AtCAT5ox-2 treated with 1 mM PPT up to 48 h (n = 8 biological replicates, except for Tak-1 at 6 and 12 h, and AtCAT5ox-2 at 6 and 48 h, where n = 3, 4, 7, and 4, respectively). Relevant data are presented in Fig. S7–S10. Source data are provided as a Source Data file.
