Fig. 5: Knockout of AtCAT1, 5, and 8, confers partial resistance to PPT and disrupts core metabolic processes.
A–C Growth of cat mutants in response to a PPT concentration gradient. Representative images of plants (A) after eight days of treatment. Estimated chlorophyll content (B) and plant size (C) were quantitated from images using ImageJ. Dose response curves were estimated using a sigmoid function and the IC50 values are listed in text of the same color. Each data point represents a single biological replicate. n = 24 for Col-0 and #7-1, except for Col-0 at 0, 50, and 100 µM. n = 12 for Salk207473. n = 6 for #8-4 and #8-5. D PPT accumulation in cat mutants after 24 h of treatment. Data points, bars, and error bars reflect individual biological replicates, mean, and standard deviation, respectively. Asterisks indicate significant differences from Col-0, as determined by one-way ANOVA and Dunnett’s post-hoc test. n = 4. E–G Basal transcriptomic profile of cat mutants showing number of dysregulated genes (E) and the enriched KEGG (F) and GO terms (G). GO terms shown are those associated with the top 20 q-values from each genotype. n = 3 biological replicates. Source data are provided as a Source Data file.
