Fig. 2: DimA promotes photo-sensing dependent behaviors.
a Quantification of lecA-lux activity in WT and mutant ΔdimA under dark and far-red light conditions. Error bars represent SEM of five biological replicates (n = 5). b Pyocyanin production (OD695) was measured and normalized to growth (OD600) in WT PA14 and the designated mutants grown under ambient light conditions. Pyocyanin levels in WT PA14 were set to 100%. Error bars represent SEM of three biological replicates (n = 3). a, b Only comparisons that had a P-value < 0.05 are denoted. Statistical significance was determined using two-way ANOVA for 1a and unpaired, two-tailed t tests in GraphPad Prism software (version 10.6.0). * P < 0.05, *** P < 0.001, **** P < 0.0001. Significant P-values were calculated as (left to right): For 2a 0.0010, for 2b < 0.0001, 0.0003, 0.0004. c Colony biofilms under dark (denoted by “black crescent” symbol), ambient (denoted by “sun” symbol) and far-red (denoted by “red bulb” symbol) for WT and mutant strains. Scale bar, 5 mm. Representative images shown of three independent experiments. d Volcano plots of RNA-seq data for WT and ΔdimA in the presence or absence of far-red light. Dark gray solid circles represent genes that are significantly repressed by far-red light by > 2-fold while mustard yellow solid circles represent genes that are significantly activated by far-red light > 2-fold. Light gray solid circles represent genes with gene expression fold changes ≥ − 2 or ≤ 2 or Padj ≥ 0.05 (P-value adjusted for multiple comparisons using the Benjamini-Hochberg procedure). e Venn diagrams showing overlaps in genes that are differentially regulated in the presence and absence of light between WT and ΔdimA mutant. Numbers indicate the number of genes differentially regulated in each intersection.
