Fig. 5: Expression of PPARγ, CD36, and FABP4/5 in tumor cells.

A PPARa, PPARd, and PPARg mRNA detected via qPCR in cells (n = 7 biological replicates). ANOVA. B PPRE binding ability for PPAR (n = 8 biological replicates). ANOVA. C, D Nuclear and cytoplasmic PPARα, PPARβ/δ, and PPARγ proteins in (C) tumor cells and (D) tumor tissues detected via Western blotting. E, F CD36, FABP4, and FABP5 proteins in (E) tumor cells and (F) tumor tissues detected via Western blotting. G CD36, FABP4, and FABP5 proteins in CT26 and DLD1 cells treated with siCtrl or siPPARg at 37 °C and 45 °C + OA were detected via Western blotting. H Detection of FA and TG levels in CT26 cells treated with siCtrl or siPparg at 37 °C and 45 °C + OA (Upper). Detection of FA and TG levels in CT26 cells treated with DMSO or T0 at 37 °C and 45 °C + OA (Lower). FA: n = 3; TG: n = 5; biological replicates. P < 0.001, ANOVA. I Detection of FA and TG levels in DLD1 cells treated with DMSO or T0 at 37 °C and 45 °C + OA (FA: n = 3, TG: n = 5, biological replicates). P < 0.001, ANOVA. J BODIPY C16 was detected in CT26 cells treated with DMSO or T0 at 37 °C and 45 °C + OA via immunofluorescence. Scale bars, 25 μm. K The positive LD area in DLD1 cells treated with DMSO or T0 at 37 °C and 45 °C + OA (n = 3 biological replicates). P < 0.001, ANOVA. L BODIPY 493 was detected in DLD1 cells treated with DMSO or T0 at 37 °C and 45 °C + OA via immunofluorescence. Scale bars, 25 μm. M,Nile red staining of CT26 cells treated with siCtrl or siPparg at 37 °C and 45 °C + OA. Data are presented as mean values ± SD. C, E and G 37/45: 37 °C/45 °C. H–L T0: T0070907. Source data are provided as a Source Data file.